LOCUS       IS26-blaSHV-IS26_unit   6114 bp    DNA     linear       03-OCT-2020
DEFINITION  Klebsiella michiganensis E718.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Klebsiella michiganensis E718
  ORGANISM  Klebsiella michiganensis E718
            Unclassified.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of IS26-blaSHV-12-IS26 unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Klebsiella michiganensis E718"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..14)
                     /gene="IRR_IS26"
     CDS             complement(53..757)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   complement(807..820)
                     /gene="IRL_IS26"
     CDS             894..1754
                     /gene="blaSHV-12"
                     /codon_start=1
                     /product="Beta-lactamase SHV-12"
                     /translation="MRYIRLCIISLLATLPLAVHASPQPLEQIKQSESQLSGRVGMIE
                     MDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYS
                     PVSEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDR
                     WETELNEALPGDARDTTTPASMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIR
                     SVLPAGWFIADKTGASKRGARGIVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGI
                     GAALIEHWQR"
     CDS             complement(1775..2536)
                     /gene="deoR"
                     /codon_start=1
                     /product="DeoR family transcriptional regulator"
                     /translation="-
                     IPIERHQRILALVEQRGAVSINELTEILGVSHMTIRRDVSKLEEQGLLVSVSGGVRAV
                     SRLAAEPSHLVKSTLQSEEKQAIGALAASHIAKNSCIYLDAGTTTLALARAILDRNDL
                     QVVTNDFEITQLLIDASQCGVIHTGGTLCRENRSCVGESAARTLRHLAIDTAFISASG
                     WDSRGIFTPDENKVTVKETVSQVSARSILLCDSSKYNQVATFMALPLTRFTTIITDRH
                     LSDAAASHIARHACEVLRAG"
     CDS             2798..3700
                     /gene="yjbJ"
                     /codon_start=1
                     /product="3-hydroxyisobutyrate dehydrogenase MmsB"
                     /translation="MAAHTNVCVIGLGSMGMGAARACLQAGLNTWGVDINPDNCRALL
                     AAGAKGAGPSAVPFAAELDAVVLLVVNAAQVRGILFGESGLAAHLKPGTVVMVSSTIA
                     SADAQAIAEALAEYQLLMLDAPVSGGAVKAAAGDMTVMASGSDAAFARLAPVLDAVAG
                     KVYRIGSDIGLGSTVKIIHQLLAGVHIAVAAEAMALAARAGIPLETMYDVVTHAAGNS
                     WMFENRMQHVLDGDYSPKSAVDIFVKDLGLVNDTARALTFPLPLATTALNMFTSASNA
                     GFGREDDSAVIKIFNGITLPGHKQ"
     CDS             3712..4344
                     /gene="yjbK"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MQLGVIADDFTGATDIASFLVRNGMPTVQLNGVPTRDIPLTSEA
                     VVISLKTRSCPAEMAVSQSLAALRWLQAQGCQQFYFKYCSTFDSTAQGNIGPVLDALL
                     AELGETRTVISPALPVNGRTVYQGYLFVGEQLLNESGMRHHPVTPMEDAHLGRLIERQ
                     GRGKAALIAWPIVARSPPRWRQSTIRRCAMWCSTPSANRICSPRAWRCGR"
     CDS             4341..4964
                     /gene="yjbL"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MKLVSGGSGLAIGLARDWAQRHGARGESAQAGMPLAGPAVVLSG
                     SCSVMTNSQVAAYRQQAPARAVDLSACFTDLESYVRTLTDWVDAQRDAPLAPMIYATT
                     EPQTLQRIQAQYGDKASSERVEQLFAALAAALKANGFTRFIVAGGETSSIVAQTLGVE
                     AFHIGPTISPGVPWVRDTRQPLSLALKSGNFGDIQFFARAQQEFRHD"
     CDS             4957..5313
                     /gene="yjbM"
                     /codon_start=1
                     /product="Class II Aldolase"
                     /translation="MTEQQLREEMVQIGASLFSRGYATGSAGNLSLLLPDGNLLATPT
                     GACLGELQAQRLSVVTLQGEWISGDKPSKEVTFHRAVYLHNPACKAIVHLHSHYLTAL
                     SCLQGLDPHNWALLQS"
     repeat_region   complement(5295..5308)
                     /gene="IRR_IS26"
     CDS             complement(5347..6051)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   complement(6101..6114)
                     /gene="IRL_IS26"
ORIGIN      
        1 ggcactgttg caaagttagc gatgaggcag ccttttgtct tattcaaagg ccttacattt
       61 caaaaactct gcttaccagg cgcatttcgc ccaggggatc accataataa aatgctgagg
      121 cctggccttt gcgtagtgca cgcatcacct caataccttt gatggtggcg taagccgtct
      181 tcatggattt aaatcccagc gtggcgccga ttatccgttt cagtttgcca tgatcgcatt
      241 caatcacgtt gttccggtac ttaatctgtc ggtgttcaac gtcagacggg caccggcctt
      301 cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc
      361 gcgggatctg ccacttcttc acgttgttga ggattttacc cagaaaccgg tatgcagctt
      421 tgctgttacg acgggaggag agataaaaat cgacagtgcg gccccggctg tcgacggccc
      481 ggtacagata cgcccagcgg ccattgacct tcacgtaggt ttcatccatg tgccacgggc
      541 aaagatcgga agggttacgc cagtaccagc gcagccgttt ttccatttca ggcgcataac
      601 gctgaaccca gcggtaaatc gtggagtgat cgacattcac tccgcgttca gccagcatct
      661 cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga
      721 tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg
      781 gatgataagt ttatcaccac cgactatttg caacagtgcc aacgccgggt tattcttatt
      841 tgtcgcttct ttactcgcct ttatcggcct tcactcaagg atgtattgtg gttatgcgtt
      901 atattcgcct gtgtattatc tccctgttag ccaccctgcc gctggcggta cacgccagcc
      961 cgcagccgct tgagcaaatt aaacaaagcg aaagccagct gtcgggccgc gtaggcatga
     1021 tagaaatgga tctggccagc ggccgcacgc tgaccgcctg gcgcgccgat gaacgctttc
     1081 ccatgatgag cacctttaaa gtagtgctct gcggcgcagt gctggcgcgg gtggatgccg
     1141 gtgacgaaca gctggagcga aagatccact atcgccagca ggatctggtg gactactcgc
     1201 cggtcagcga aaaacacctt gccgacggca tgacggtcgg cgaactctgc gccgccgcca
     1261 ttaccatgag cgataacagc gccgccaatc tgctgctggc caccgtcggc ggccccgcag
     1321 gattgactgc ctttttgcgc cagatcggcg acaacgtcac ccgccttgac cgctgggaaa
     1381 cggaactgaa tgaggcgctt cccggcgacg cccgcgacac cactaccccg gccagcatgg
     1441 ccgcgaccct gcgcaagctg ctgaccagcc agcgtctgag cgcccgttcg caacggcagc
     1501 tgctgcagtg gatggtggac gatcgggtcg ccggaccgtt gatccgctcc gtgctgccgg
     1561 cgggctggtt tatcgccgat aagaccggag ctagcaagcg gggtgcgcgc gggattgtcg
     1621 ccctgcttgg cccgaataac aaagcagagc gcattgtggt gatttatctg cgggatacgc
     1681 cggcgagcat ggccgagcga aatcagcaaa tcgccgggat cggcgcggcg ctgatcgagc
     1741 actggcaacg ctaagccggc ggtggccgcg cgcgttatcc ggcccgcagc acctcgcagg
     1801 cgtgccgggc gatatgactg gcggcggcat cggaaagatg ccggtcggta atgatggtgg
     1861 tgaaccgggt caaaggtaac gccataaacg tggccacctg attgtatttc gaactgtcgc
     1921 acagcaggat gcttctcgcg ctgacctggc tgacggtctc cttgacggta accttgttct
     1981 catcaggggt gaatatcccg cgactgtccc agccgctggc ggagataaag gccgtatcga
     2041 tagccaggtg gcgtaacgta cgcgccgccg attcgcccac gcaggagcgg ttctcccggc
     2101 acagagtgcc gccggtgtgg atcacgccgc actggctggc atcgatcagc agctgggtta
     2161 tctcaaaatc attggtgacc acctggagat cgttccggtc gaggatcgcc cgcgccagcg
     2221 ccagggtggt agtcccggca tccagataga tgcaactgtt tttagcgata tgactcgccg
     2281 ccagcgcgcc gatcgcctgt ttctcctcac tctgcagcgt gcttttcacc agatgactgg
     2341 gttccgcggc cagccggctg acggcgcgta cgccgcccga gacgctgacc agcagcccct
     2401 gctcctccag tttactgacg tcccgacgga tggtcatatg ggacacgccg aggatctccg
     2461 tcagctcgtt aatgcttacc gccccacgct gctccaccag ggctaaaata cgctgatgtc
     2521 gttctattgg aatcaccgct ctccccttac cattttttca caccaggcgt caccacccag
     2581 gctacggccc tggcgacacc cggtgttgtt atcgcgcttt gccgctgttt tttacgctat
     2641 tttagggcaa gaatcgccgc tgtgcagcct ctttccgcct gtgaattttt tatattcatg
     2701 tgggttattc gtgataactc tcacataaat tcacatgata acgctttatt ctatcattaa
     2761 atcacattaa ttaactaatg ttcacaagga gaccagcatg gctgcacaca ctaacgtctg
     2821 cgtgattgga ctgggttcaa tgggcatggg cgccgcccgc gcctgcctgc aggcgggcct
     2881 gaacacctgg ggcgttgaca tcaatcccga caactgtcgc gcactgctgg cggcgggcgc
     2941 caaaggcgcg ggccccagcg cggtgccgtt cgccgcggaa ctggatgcag ttgtgctgct
     3001 ggtggtcaat gccgcccagg tgcgggggat cctgttcggc gagagcggcc tcgccgccca
     3061 tctgaagccg ggcaccgtcg tgatggtgtc gtccaccatc gcctccgccg atgctcaggc
     3121 cattgccgag gcgctggcgg agtaccagct attgatgctc gacgcgccgg tatcgggcgg
     3181 cgccgtgaaa gcggccgccg gcgacatgac ggtgatggcc tccgggagcg atgccgcctt
     3241 tgcccgactc gcgccggtgc tggacgccgt ggccggcaaa gtctaccgca tcgggagcga
     3301 cattggtctt ggctcgacgg taaaaattat ccatcagctg ctggccgggg tgcacatcgc
     3361 cgttgccgcc gaagcgatgg cgcttgccgc ccgcgccggg atcccactgg agacgatgta
     3421 tgacgtggtc acccacgcgg cgggtaattc ctggatgttt gagaatcgca tgcagcacgt
     3481 cctggatggc gattactcgc caaaatccgc tgtcgatatt tttgtcaaag atctcgggct
     3541 ggtgaatgac actgcccggg cgctgacctt cccgctgccg ctcgctacca ccgcgctgaa
     3601 tatgttcacc tccgccagta atgccggatt cggtcgggaa gatgacagcg cggtgatcaa
     3661 gattttcaac ggcatcaccc tgccgggcca taaacagtga ggagagacaa catgcagctt
     3721 ggtgtcattg ccgatgactt caccggcgcc acggatattg ccagcttcct cgtgcgcaac
     3781 ggcatgccga cggtgcaact gaatggcgtg ccgacccgcg atattccgct gaccagcgag
     3841 gcggtggtca tcagcctgaa aacccgctcc tgcccggcgg aaatggccgt cagccagtcg
     3901 ctggcggccc tgcgctggct gcaggcccag ggctgtcagc agttttattt caagtactgt
     3961 tccactttcg acagcaccgc gcagggcaac attggcccgg tgctggatgc cctgctggcc
     4021 gagctgggtg agacgcggac ggtgatttcc ccggcgctgc cggttaacgg ccgcacggtc
     4081 tatcagggat atctgttcgt cggcgagcaa ctgctgaatg agtccgggat gcgccaccat
     4141 ccggtgacgc cgatggagga tgcgcacctg ggccgcttaa ttgagcgcca agggcgcgga
     4201 aaagccgcgc tgattgcctg gccgattgtc gcccggtcgc cgccgcgctg gcggcagtca
     4261 acgatccggc ggtgcgctat gtggtgctcg acgccctcag cgaacaggat ctgctcaccc
     4321 agggcgtggc gctgcgggag atgaagctgg tctccggcgg ttccggcctc gccatcggcc
     4381 tcgcccgcga ctgggcgcag cgccatggcg ccagggggga aagcgctcag gccggcatgc
     4441 cgctggccgg tccggcggtg gtgctctcgg gctcctgctc ggtgatgacc aacagccagg
     4501 tggcggccta tcgtcaacag gcccccgccc gcgccgtcga cttaagcgcc tgctttaccg
     4561 atctggagag ctacgtcagg acgctgactg actgggtgga cgcgcagcgc gatgcgccgc
     4621 tggcgccgat gatctatgcc accaccgagc cgcaaacgct gcagcggatc caggcgcagt
     4681 atggcgacaa ggccagcagc gaacgggtgg aacagctgtt tgccgctctt gccgccgccc
     4741 tgaaggcgaa cggatttacc cgctttattg tggccggagg ggaaacgtcg agcattgtgg
     4801 cgcagaccct gggggttgag gcgttccata ttgggccgac catctcccct ggcgtgccct
     4861 gggtgcgtga cacccgccag ccgctctccc tggcgctgaa gtcaggtaac ttcggcgata
     4921 tccagttctt tgcccgtgcc cagcaggagt ttcgtcatga ctgagcaaca actgcgagag
     4981 gaaatggtgc agattggcgc ctcgttgttt agccgcggct atgccaccgg ctccgctggc
     5041 aatctgtcgc tgctgctgcc ggacggcaac ctgctggcga cgccgaccgg cgcctgcctc
     5101 ggcgaactgc aggctcagcg gttgtcggtg gtgacgctgc aaggggaatg gatctccggc
     5161 gacaaaccgt cgaaagaggt cacttttcac cgggcggtct atttgcacaa cccggcctgc
     5221 aaggcgatcg tccacttgca cagccactat ctgaccgcgc tctcctgcct gcaggggctc
     5281 gatccgcaca actgggcact gttgcaaagt tagcgatgag gcagcctttt gtcttattca
     5341 aaggccttac atttcaaaaa ctctgcttac caggcgcatt tcgcccaggg gatcaccata
     5401 ataaaatgct gaggcctggc ctttgcgtag tgcacgcatc acctcaatac ctttgatggt
     5461 ggcgtaagcc gtcttcatgg atttaaatcc cagcgtggcg ccgattatcc gtttcagttt
     5521 gccatgatcg cattcaatca cgttgttccg gtacttaatc tgtcggtgtt caacgtcaga
     5581 cgggcaccgg ccttcgcgtt tgagcagagc aagcgcgcga ccataggcgg gcgctttatc
     5641 cgtgttgatg aatcgcggga tctgccactt cttcacgttg ttgaggattt tacccagaaa
     5701 ccggtatgca gctttgctgt tacgacggga ggagagataa aaatcgacag tgcggccccg
     5761 gctgtcgacg gcccggtaca gatacgccca gcggccattg accttcacgt aggtttcatc
     5821 catgtgccac gggcaaagat cggaagggtt acgccagtac cagcgcagcc gtttttccat
     5881 ttcaggcgca taacgctgaa cccagcggta aatcgtggag tgatcgacat tcactccgcg
     5941 ttcagccagc atctcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     6001 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     6061 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgcc
//
